Microplate readers, multi-mode, SpectraMax® i3x. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA) using the setup of excitation and emission provided in the SoftMax Pro 7. 6% for the on-board optics (monochromators). It allows the user to determine the total concentration of protein in a solution. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. Get Pricing. SpectraMax i3x Multi-Mode Microplate Reader System. The calculated LOQ values were 0. polarization performance of the FilterMax®, SpectraMax® Paradigm®, and select cartridges or read modes of the SpectraMax i3, i3x, and iD5 readers. VAL014, right table) assays on the SpectraMax i3x reader are shown below. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreSimilar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalSpectraMax i3x Multi-Mode Microplate Reader. Automated for ease of use. Every SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. Molecular Devices are known for both the reliability and the wide uses of their products. Meanwhile, the samples were detected with a fluorometric reader Spectra Max i3x (MD, America) at 488 nm excitation wavelength and 525 nm emission wavelength. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. The Molecular Devices SpectraMax i3x is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. i3. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. SpectraMax M 시리즈 Microplate Reader, Cuvette Port, IQ OQ 검증 | Molecular Devices. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. ). Lower limits of detection for ATP determined using the ATPlite . Molecular Devices are known for both the reliability and the wide uses of their products. Next,. infoboxeu@moldev. Spectranet customer portalThe Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). Expand your lab’s capabilities to include flash applications such as dual luciferase and. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. pathways and protein activation and expression in one system. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. SCGPU05RE)A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Enzyme Activation Analysis;. 2. Robust,. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Hands-on fun and learning that empowers exploring, inventing and sharing. SpectraMax iD5:Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. Flow cytometry was used to measure ROS concentrations, based on fluorescence intensity (FL-1, 530 nm) of 10,000 cells. 22 μ m fi ltration unit (Sigma, cat. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 6% for the on-board optics (monochromators). The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). ) wide and 65 cm (25. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 1. The absorbance at 450 nm was read with a Spectra Max i3X microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA), and the data at 30–40 min post-treatment were analyzed. ATP standard curves read on SpectraMax microplate readers. Figure 4. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. SpectraMax i3x. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. Replicate values were averaged and imported into Design-Expert 8. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. SpectraMax i3x, onboard luminescence 121. 在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. A mixture of 6-nitro-1H,3H-benzo[de]isochromene-1,3-dione (486 mg, 2. SpectraMax i3/i3x readers must be equipped with a suitableWhen we measured the FRET ratio using the Spectramax i3x plate reader, we found that FRET ratios were higher in DMSO-treated cells that had not been incubated at room temperature to enhance fluorescence than in room temperature-incubated cells (Fig. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 305 reference level saturation. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 価格、見積もり、お近くの販売店を. 발광 (Luminescence) 리더기. Contact Us Phone: +1. 8 Antibodies and. Find regional support. 312 gain calibration failed. About the reader setup recommendations The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Spectranet customer portalThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 제품. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. g. Example: [Drawer]. Description 1. 0001 0. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 0. *On the SpectraMax i3x reader only. Assay setup for a 384-well low-volume plate. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. Equipped with transmitted light (TL), green (Ex/Em: 460/541) and red (625/713) fluorescence channels, the MiniMax cytometer with SoftMax® Pro Software can be used to image and. We would like to show you a description here but the site won’t allow us. High resolution X-ray photoelectron spectroscopy (XPS) of cCQD was obtained on a K-Alpha+ (Thermo Fisher Scientific, Massachusetts, USA) spectrometer. , 2021). HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). Home > Search Results > Danaher Inc > spectramax i3x. . The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Thetypeof luciferase(Renilla. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Detection of pro-inflammatory factors. Download PDF. The SpectraMax i3x reader provides the With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using. Multimode Reader Comparison Chart. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax i2X is used for cell migration, cell. 在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. 58% for the detection module and 2. 吸収度マイクロプレートリーダー SpectraMax® Mini. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The treatments were. 吸収度マイクロプレートリーダー SpectraMax® Mini. Find regional support. The fluorescence spectra were acquired using a microplate reader (Spectra Max i3x; Molecular Devices, USA). This way you’ll save time on finding the necessary info. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Nuclear Receptor. Kastelic,2 and Jian Gao 1 1Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Features. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyWe would like to show you a description here but the site won’t allow us. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SpectraMax i3x Multi-Mode Detection Platform. Synthesis of ZM-1NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Order Now. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Introduction. Examples: fiPress carefully to engage,fl fiDo not press down. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 01 0. Example: [Drawer]. , 2021). The size and fluorescence intensity were tested for 48 h. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. Immunofluorescence StainingAfter cooled down to room temperature, the concentrations of the solution were measured by microplate reader (Spectra Max i3x) at 490 nm (Kagi and Schaffer, 1988). Fluorescence Microplate Reader Comparison Chart. Research Article Streptococcus lutetiensis Induces Autophagy via Oxidative Stress in Bovine Mammary Epithelial Cells Peng Chen ,1 Jingyue Yang,1 Naiwen Wu,1 Bo Han,1 John P. The SpectraMax i3x reader provides the. each new assay. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. As. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. ) deep, preferably with access on all sides. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Molecular Devices Corporation. The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. Data Analysis. ZERO BIAS - scores, article reviews, protocol conditions and moreWe would like to show you a description here but the site won’t allow us. 800. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a 96-well black, clear-bottom microplate. Find regional support. Capture Flash Assays with Ease. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. flThe SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Prepare your i3/i3x instrument for shipment. Introduction. Key features. Downloads. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The cell imaging was captured on Zeiss LSM 980 confocal microscopy. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. 11. Microplate optimization should be performed the first time the assay is run, and subsequently for . Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. Detection of pro-inflammatory factors. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6 - 384 well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. SpectraMax i3, i3x, and iD5 readers. 0´ 104 1. The SpectraMax i3 also allows the user to identify antibodies during Elisa Assays. Promega. Increased fluorescence performance. Specifically, we present neurite. The chamber. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. i3. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. Prime function 20 amol. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Examples: fiPress carefully to engage,fl fiDo not press down. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. Published Dec 16, 2020 | Updated Oct 10, 2023. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. 315 can't find zero order. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. . The ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. 3, were mixed in a final volume of 100 μL in 10 mM sodium phosphate buffer, pH 7. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely low light. 0. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). The SoftMax Pro Software provides integrated instrument control. Prismatic colour and motion for everyone. The plate was covered and incubated for one hour at room temperature. 7 mm for all shake intensities. 0. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. Plate chamber design of the SpectraMax category 1 microplate readers. The fluorescence spectra was obtained on a Spectra Max i3x Multi-Mode Detection Platform. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Fluorescence was measured using a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA) with 365 nm excitation and 450 nm emission filters. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. Promega. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. Prepare your i3/i3x instrument for shipment. To learn more about the device, please watch this short video. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. Recommended settings for SpectraMax iD5 and i3x readers. Description 1. We would like to show you a description here but the site won’t allow us. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. Supplier Page. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Introduction. Actual shake speed is based on the microplate format. Italic and boldface type are used for emphasis. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 3: The cuvette chamber. SpectraMax Microplate Readers. 00800 665 32860. 303 unable to cal dark current. In dual reporter gene assays, cells are transfected with two vectors, the first containing an experimental reporter gene coupled to a regulated promoter of interest, andSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. With optimized reagents, option to operate the readers. These robust readers have been placed in labs from Antarctica to the. Also refer to this Multimode Reader Comparison Chart. The SpectraMax ® i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. Find regional support. no. 316 grating motor driver faulty. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . 305 reference level saturation. How to properly unlock iD series instrument. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. 1. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. However, no piece of equipment is immune to breaking down. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. Patented user-exchangeable cartridge design expands the system's detection capability making it. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. 価格、見積もり、お近くの販売店を. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength and superior sensitivity across the full. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. applications, including dual luciferase and ATP assays. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using a Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA, USA) with the excitation and emission setup provided with SoftMax Pro 7. Figure 2-23: Bottle holder To install the bottle holder: 1. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthCapture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. Singlet oxygen (1 O 2) generation was determined through 1,3-diphenylisobenzofuran (DPBF) quenching. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax MiniMax 300 イメージングサイトメーター は、研究室でのアップグレードが可能なSpectraMax i3xシステム専用のオプションで、ユーザーは複雑なイメージングシステムに投資することなく、細胞増殖を解析できます。SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. i3x. 2 only) SpectraMax M-series Microplate readers. Fourier transform infrared (FT-IR) spectra of cCQD were obtained on a. The protocols are also available on the SoftMax Pro Software protocol sharing. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). 7. The assay can accurately test the toxicology of compounds as shown in Figure 1. ) wide and 65 cm (25. Turbidity measurements were performed with a Spectra Max® i3x microplate reader to study the effect of oxidation on the kinetics of sFN formation. 314 low lamp level warning. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength*On the SpectraMax i3x reader only. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. +44 118 944 8000. SpectraMax i3x Multi-Mode Microplate Reader SystemSpectraMax® i3x dari Molecular Devices adalah sistem deteksi berbasis multi-mode yang berevolusi menyesuaik. Prepare your i3/i3x instrument for shipment. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. HTRF IP standard curve. HTRF-compatible SpectraMax Multi-Mode Readers. Patented user-exchangeable cartridge design expands the system's detection capability making it. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. applications, including dual luciferase and ATP assays. D. com. TUNE Tunable Wavelength Detection Cartridge. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. The chamber. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. The SoftMax Pro Software quickly. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthSpectraMaxABSInstrumentInstallationGuide 4 5065391A ComputerIntegration EachMolecularDevicesmicroplatereaderisshippedwithalicensekeyfortheSoftMax®ProSpectraMax iD5 reader. The UV-Vis spectroscopy was measured on a TU-1900Beijing PuXi instrument. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. If you do not have an iLab account you must first make one: SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. The SPECTRAmax 340PC can mea-Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. 99（图一）。After incubation at room temperature for one hour in the dark, we measured the fluorescence intensity using a SpectraMax i3x (Molecular Devices, CA, USA), with excitation at 350 nm and dual emissions at 435 nm and 490 nm. As. Increased fluorescence performance. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. 12911151) 0. SpectraMax® iD3/iD5. Price from $9. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. Explore a wealth of applications with the future-ready SpectraMax i3x. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Explore a wealth of applications in one future-ready system that measures spectral-based absorbance, fluorescence, and luminescence. QBT Fatty Acid Transporter Assay. Molecular Devicesの全商品を見る. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). However, no piece of equipment is immune to breaking down. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Fluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. 001 0. Eliminate time-dependent substrate addition steps. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. SpectraMax i3x. *On the SpectraMax i3x reader only. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Simplified workflow: Add cellular imaging assays in a small footprint. 2 (Molecular Devices, San Jose, CA, USA). Fluorescence was measured using a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA) with 365 nm excitation and 450 nm emission filters. Open the catalog to page 1. Molecular Devicesの全商品を見る.